Co-expression of the C-terminal domain of Yersinia enterocolitica invasin enhances the efficacy of classical swine-fever-vectored vaccine based on human adenovirus

Journal of biosciences

Lei He²  Yanming Zhang1¹  Helin Li¹  Kai Kang¹  Wulong Liang¹  Zhi Lin¹  Pengbo Ning¹ 

[1] College of Veterinary Medicine, Northwest A & F University, Yangling 712100, Shaanxi, China$$;College of Animal Science & Technology, Henan University of Science and Technology, Luoyang 471023, Henan, China$$

关键词: Classical swine fever (CSF);    E2;    human adenovirus;    invasin;    vaccine;   

DOI  :  

来源: Indian Academy of Sciences

【 摘 要 】

The use of adenovirus vector-based vaccines is a promising approach for generating antigen-specific immune responses. Improving vaccine potency is necessary in other approaches to address their inadequate protection for the majority of infectious diseases. This study is the first to reconstruct a recombinant replication-defective human adenovirus co-expressing E2 and invasin C-terminal (InvC) glycoproteins (rAd-E2-InvC). rAd-E2-InvC with 2×106 TCID50 was intramuscularly administered two times to CSFV-free pigs at 14 day intervals. No adverse clinical reactions were observed in any of the pigs after the vaccination. The CSFV E2-specific antibody titer was significantly higher in the rAd-E2-InvC group than that in the rAdV-E2 group as measured by NPLA and blocking ELISA. Pigs immunized with rAd-E2-InvC were completely protected against lethal challenge. Neither CSFV RNA nor pathological changes were detected in the tissues after CSFV challenge. These results demonstrate that rAd-E2-InvC could be an alternative to the existing CSF vaccine. Moreover, InvC that acts as an adjuvant could enhance the immunogenicity of rAdV-E2 and induce high CSFV E2-specific antibody titer and protection level.

【 授权许可】

Unknown