Phosphorylation of DegU is essential for activation of amyE expression in Bacillus subtilis

Journal of biosciences

Monica Gupta1 2¹  K Krishnamurthy Rao¹ 

[1] Gene Regulation Lab, Department of Biosciences and Bioengineering, Indian Institute of Technology (IIT) Bombay, Powai, Mumbai 400 076, India$$

关键词: 𝛼-Amylase;    amyE;    B. subtilis;    DegU-P;    expression;    promoter activity;   

DOI  :  

来源: Indian Academy of Sciences

【 摘 要 】

Alpha (𝛼)-amylase (amyE) is one of the major exo-enzymes secreted by Bacillus subtilis during the post-exponential phase. The DegS-DegU two-component system regulates expression of majority of post-exponentially expressed genes in B. subtilis. It has been demonstrated that varying levels of the phosphorylated form of DegU (DegU-P) control different cellular processes. Exo-protease production is observed when effective concentration of DegU-P rises in the cell, whereas swarming motility is favoured at very low amounts of DegU-P. In this study we show that like other exo-proteases, expression of amyE is positively regulated by increase in DegU-P levels in the cell. We also demonstrate that residues at the DNA-binding helix-turn-helix (HTH) motif of DegU are necessary for the amyE expression. This observation is further reinforced by demonstrating the direct interaction of DegU on amyE promoter.

【 授权许可】

Unknown