Excised radicle tips as a source of genomic DNA for PCR-based genotyping and melting curve analysis in cotton

Journal of biosciences

P Srinivasa Rao²  Ramesh V Sonti1²  P Sateesh Kumar¹ 

[1] Nuziveedu Seeds, Road No. 12, Banjara Hills, Hyderabad 500 034, India$$;Centre for Cellular and Molecular Biology (CCMB), Uppal Road, Hyderabad 500 007, India$$

关键词: Cotton;    cry1Ac;    genomic DNA isolation;    high-resolution melting curve analysis;    radicle tip;    seed purity testing;   

DOI  :  

来源: Indian Academy of Sciences

【 摘 要 】

Genomic DNA isolation in cotton is complicated because of the presence of secondary metabolites that are inhibitory to PCR amplification. We report here that radicle tips, but not other parts of cotton seedlings, yield high-quality DNA that is readily amenable for PCR. The radicle-tip-excised seedlings retain viability because of the formation of adventitious roots. We demonstrate the utility of this method in distinguishing homozygotes from heterozygotes in a cotton breeding population and in hybrid seed purity testing.

【 授权许可】

Unknown