Evaluation of the DNA damaging effects of amitraz on human lymphocytes in the Comet assay

Journal of biosciences

Ninoslav Djelic1²  Branka Vukovic-Gacic³  Jevrosima Stevanovic²  Zoran Stanimirovic²  Nada Lakic¹  Jelena Knezevic-Vukcevic³  Milena Radakovic² 

[1] Department of Statistics, Faculty of Agriculture, The University of Belgrade, Belgrade, Serbia$$;Department of Biology, Faculty of Veterinary Medicine, The University of Belgrade, Belgrade, Serbia$$;Department of Microbiology, Faculty of Biology, The University of Belgrade, Belgrade, Serbia$$

关键词: Amitraz;    apoptosis;    catalase;    Comet assay;    DNA damage;   

DOI  :  

来源: Indian Academy of Sciences

【 摘 要 】

Amitraz is formamidine pesticide widely used as insecticide and acaricide. In veterinary medicine, amitraz has important uses against ticks, mites and lice on animals. Also, amitraz is used in apiculture to control Varroa destructor. It this study, the alkaline Comet assay was used to evaluate DNA damaging effects of amitraz in human lymphocytes. Isolated human lymphocytes were incubated with varying concentrations of amitraz (0.035, 0.35, 3.5, 35 and 350 𝜇g/mL). The Comet assay demonstrated that all concentrations of amitraz caused statistically significant increase in the level of DNA damage, thus indicating that amitraz possesses genotoxic potential. The concentration of amitraz that produced the highest DNA damage (3.5 𝜇g/mL) was chosen for further analysis with the antioxidant catalase. The obtained results showed that co-treatment with antioxidant catalase (100 IU/mL or 500 IU/mL) significantly reduced the level of DNA damage, indicating the possible involvement of reactive oxygen species in DNA damaging effects of amitraz. Flow cytometric analysis revealed increase of the apoptotic index following treatment with amitraz. However, co-treatment with catalase reduced the apoptotic index, while treatment with catalase alone reduced the percentage of apoptotoc cells even in comparison with the negative control. Therefore, catalase had protective effects against ROS-mediated DNA damage and apoptosis.

【 授权许可】

Unknown