Enantioselective hydrolysis of epichlorohydrin using whole Aspergillus niger ZJB-09173 cells in organic solvents

Journal of biosciences

Huo-Xi Jin¹  Yu-Guo Zheng1 2¹  Zhong-Ce Hu¹ 

[1] Institute of Bioengineering, Zhejiang University of Technology, Hangzhou 310014, China$$

关键词: Enantioselective hydrolysis;    enantioselectivity;    epichlorohydrin;    epoxide hydrolase;    organic solvent;   

DOI  :  

来源: Indian Academy of Sciences

【 摘 要 】

The enantioselective hydrolysis of racemic epichlorohydrin for the production of enantiopure (𝑆)-epichloro-hydrin using whole cells of Aspergillus niger ZJB-09173 in organic solvents was investigated. Cyclohexane was used as the reaction medium based on the excellent enantioselectivity of epoxide hydrolase from A. niger ZJB-09173 in cyclohexane. However, cyclohexane had a negative effect on the stability of epoxide hydrolase from A. niger ZJB-09173. In the cyclohexane medium, substrate inhibition, rather than product inhibition of catalysis, was observed in the hydrolysis of racemic epichlorohydrin using A. niger ZJB-09173. The racemic epichlorohydrin concentration was markedly increased by continuous feeding of substrate without significant decline of the yield. Ultimately, 18.5% of (𝑆)-epichlorohydrin with 98% enantiomeric excess from 153.6 mM of racemic epichlorohydrin was obtained by the dry cells of A. niger ZJB-09173, which was the highest substrate concentration in the production of enantiopure (𝑆)-epichlorohydrin by epoxide hydrolases using an organic solvent medium among the known reports.

【 授权许可】

Unknown