Molecular analysis of gut microbiota in obesity among Indian individuals

Journal of biosciences

Dhiraj P Dhotre²  Dhawal S Jain¹  Jayshree Gangawani⁴  Shashank Shah⁴  Vikram B Lanjekar³  Sachin G Chavan²  Poonam S Shah⁴  Yogesh S Shouche1²  Milind S Patole²  Armiya Sultan²  Dilip R Ranade³  Deepak P Patil²  Jayshree S Todkar⁴ 

[1] Institute of Bioinformatics and Biotechnology, University of Pune, Pune 411 007, India$$;National Centre for Cell Science, Ganeshkhind, Pune 411 007, India$$;Microbial Sciences Division, Agharkar Research Institute, Pune 411 004, India$$;Laparo Obeso Centre, Ruby Hall Clinic, Sassoon Road, Pune 411 001, India$$

关键词: Gut microbiota;    Indian;    microbial diversity;    obesity;   

DOI  :  

来源: Indian Academy of Sciences

【 摘 要 】

Obesity is a consequence of a complex interplay between the host genome and the prevalent obesogenic factors among the modern communities. The role of gut microbiota in the pathogenesis of the disorder was recently discovered; however, 16S-rRNA-based surveys revealed compelling but community-specific data. Considering this, despite unique diets, dietary habits and an uprising trend in obesity, the Indian counterparts are poorly studied. Here, we report a comparative analysis and quantification of dominant gut microbiota of lean, normal, obese and surgically treated obese individuals of Indian origin. Representative gut microbial diversity was assessed by sequencing fecal 16S rRNA libraries for each group (n=5) with a total of over 3000 sequences. We detected no evident trend in the distribution of the predominant bacterial phyla, Bacteroidetes and Firmicutes. At the genus level, the bacteria of genus Bacteroides were prominent among the obese individuals, which was further confirmed by qPCR (𝑃 > 0.05). In addition, a remarkably high archaeal density with elevated fecal SCFA levels was also noted in the obese group. On the contrary, the treated-obese individuals exhibited comparatively reduced Bacteroides and archaeal counts along with reduced fecal SCFAs. In conclusion, the study successfully identified a representative microbial diversity in the Indian subjects and demonstrated the prominence of certain bacterial groups in obese individuals; nevertheless, further studies are essential to understand their role in obesity.

【 授权许可】

Unknown