Aggregation properties of a short peptide that mediates amyloid fibril formation in model proteins unrelated to disease

Journal of biosciences

Nitin Chaudhary¹  Shashi Singh¹  Ramakrishnan Nagaraj1¹ 

[1] CSIR-Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India$$

关键词: Amyloid fibrils;    atomic force microscopy;    non-fibrillar structures;    peptide self-association;    SH3 domain;   

DOI  :  

来源: Indian Academy of Sciences

【 摘 要 】

Short peptides have been identified from amyloidogenic proteins that form amyloid fibrils in isolation. The hexapeptide stretch 21DIDLHL26 has been shown to be important in the self-assembly of the Src homology 3 (SH3) domain of p85𝛼 subunit of bovine phosphatidylinositol-3-kinase (PI3-SH3). The SH3 domain of chicken brain 𝛼-spectrin, which is otherwise non-amyloidogenic, is rendered amyloidogenic if 22EVTMKK27 is replaced by DIDLHL. In this article, we describe the aggregation behaviour of DIDLHL-COOH and DIDLHL-CONH2. Our results indicate that DIDLHL-COOH and DIDLHL-CONH2 aggregate to form spherical structures at pH 5 and 6. At pH 5, in the presence of mica, DIDLHL-CONH2 forms short fibrous structures. The presence of NaCl along with mica results in fibrillar structures. At pH 6, DIDLHL-CONH2 forms largely spherical aggregates. Both the peptides are unstructured in solution but adopt 𝛽-conformation on drying. The aggregates formed by DIDLHL-COOH and DIDLHL-CONH2 are formed during drying process and their structures are modulated by the presence of mica and salt. Our study suggests that a peptide need not have intrinsic amyloidogenic propensity to facilitate the selfassembly of the full-length protein. The propensity of peptides to form self-assembled structures that are non-amyloidogenic could be important in potentiating the self-assembly of full-length proteins into amyloid fibrils.

【 授权许可】

Unknown