The Chriz–Z4 complex recruits JIL-1 to polytene chromosomes, a requirement for interband-specific phosphorylation of H3S10

Journal of biosciences

Miao Gan¹  Selina Moebus¹  Harald Eggert¹  Harald Saumweber1¹ 

[1] Humboldt University Berlin, Institute for Biology, Cytogenetics Section, Chausseestr. 117, D10115 Berlin, Germany$$

关键词: ChIP;    chromodomain;    immunoprecipitation;    zinc-finger;   

DOI  :  

来源: Indian Academy of Sciences

【 摘 要 】

The conserved band-interband pattern is thought to reflect the looped-domain organization of insect polytene chromosomes. Previously, we have shown that the chromodomain protein Chriz and the zinc-finger protein Z4 are essentially required for the maintenance of polytene chromosome structure. Here we show that both proteins form a complex that recruits the JIL-1 kinase to polytene chromosomes, enabling local H3S10 phosphorylation of interband nucleosomal histones. Interband targeting domains were identified at the N-terminal regions of Chriz and Z4, and our data suggest partial cooperation of the complex with the BEAF boundary element protein in polytene and diploid cells. Reducing the core component Chriz by RNAi results in destabilization of the complex and a strong reduction of interband-specific histone H3S10 phosphorylation.

【 授权许可】

Unknown