Towards hemerythrin-based blood substitutes: Comparative performance to hemoglobin on human leukocytes and umbilical vein endothelial cells

Journal of biosciences

Augustin Mot²  Florina Deac²  Radu Silaghi-Dumitrescu2²  Eva Fischer-Fodor¹  Mariann Arkosi² 

[1] Ion Chiricuta Cancer Institute – Comprehensive Cancer Center, 34–36 Republicii St, Cluj-Napoca 400015, Romania$$;Department of Chemistry, Babes-Bolyai University, 11 Arany Janos Street, Cluj-Napoca 400028, Romania$$

关键词: Blood substitute;    hemoglobin;    hemerythrin;    human umbilical vein endothelial cell (HUVEC);    lymphocyte;   

DOI  :  

来源: Indian Academy of Sciences

【 摘 要 】

Hemerythrin is a dioxygen-carrying protein whose oxidative/nitrosative stress-related reactivity is lower than that of hemoglobin, which may warrant investigation of hemerythrin as raw material for artificial oxygen carriers (‘blood substitutes’). We report here the first biological tests for hemerythrin and its chemical derivatives, comparing their performance with that of a representative competitor, glutaraldehyde-polymerized bovine hemoglobin. Hemerythrin (native or derivatized) exhibits a proliferative effect on human umbilical vein endothelial cell (HUVEC) cultures, as opposed to a slight inhibitory effect of hemoglobin. A similar positive effect is displayed on human lymphocytes by glutaraldehyde-polymerized hemerythrin, but not by native or polyethylene glycol-derivatized hemerythrin.

【 授权许可】

Unknown