Proteomic analysis of heparin-binding proteins from human seminal plasma: a step towards identification of molecular markers of male fertility

Journal of biosciences

Tej P Singh²  Jaya Nautiyal²  Vijay Kumar²  Savita Yadav1²  Anil Kumar Tomar²  Sarman Singh¹  Md Imtaiyaz Hassan²  Tara Kashav³ 

[1] Department of Lab Medicine, All India Institute of Medical Sciences, New Delhi 110 029, India$$;Department of Biophysics, All India Institute of Medical Sciences, New Delhi 110 029, India$$;School of Life Sciences, Jawaharlal Nehru University, New Delhi 110 067, India$$

关键词: 2D gel electrophoresis;    human seminal plasma;    heparin-binding proteins;    MALDI-TOF;    sperm capacitation;   

DOI  :  

来源: Indian Academy of Sciences

【 摘 要 】

Glycosaminoglycans, especially heparin, are involved in various cell processes such as apoptosis, cell cycle control, platelet activation, capacitation, acrosome reaction and sperm decondensation. Heparin-binding proteins (HBPs) are essential constituents of human seminal fluid, which bind to sperm lipids containing the phosphorylcholine group and mediate the fertilization process. We utilized a proteomic set-up consisting of affinity chromatography, isoelectric focusing (IEF) coupled with matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry (MALDI TOF/MS) for protein analysis of human HBPs. We resolved 70 different spots on two-dimensional (2-D) gel and subsequently identified these proteins. Forty different types of proteins were identified. Functional analysis revealed that 38% of the proteins belonged to the enzyme category, 20% were involved in RNA processing and transcription, 18% in structure and transport function, and 16% in cell recognition and signal transduction. We also identified 8% of proteins with unknown functions, although their expression in seminal fluid has been documented. Proteins of seminal fluid that bind heparin may be directly involved in sperm capacitation and acrosome reaction (AR), which are the two critical steps for fertilization. This information on HBPs would be useful for identifying potential biomarkers of fertility in the near future.

【 授权许可】

Unknown