Nano-composition of riboflavin–nafion functional film and its application in biosensing

Journal of biosciences

A Bayandori-Moghaddam³  S Ahmadiam¹  A Barzegar¹  S Rezaei-Zarchi¹  A A Saboury1¹  A Javed² 

[1] Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran$$;Department of Biochemistry, University of Agriculture, Faisalabad, Pakistan$$;Department of Chemistry, Faculty of Sciences, University of Tehran, Tehran, Iran$$

关键词: Biosensor;    functional membrane;    horseradish peroxidase;    nanoparticles;   

DOI  :  

来源: Indian Academy of Sciences

【 摘 要 】

A novel nafion–riboflavin membrane was constructed and characterized by the scanning electron microscopy (SEM), transmission electron microscopy (TEM), UV-visible spectroscopy and cyclic voltammetric techniques. The estimated average diameter of the designed nanoparticles was about 60 nm. The functional membrane showed a quasi-reversible electrochemical behaviour with a formal potential of –562 ± 5 mV (vs Ag/AgCl) on the gold electrode. Some electrochemical parameters were estimated, indicating that the system has good and stable electron transfer properties. Moreover, horseradish peroxidase (HRP) was immobilized on the riboflavin–nafion functional membrane. The electrochemical behaviour of HRP was quasi-reversible with a formal potential of 80 ± 5 mV (vs Ag/AgCl). The HRP in the film exhibited good catalytic activity towards the reduction of H2O2. It shows a linear dependence of its cathodic peak current on the concentration of H2O2, ranging from 10 to 300 𝜇M.

【 授权许可】

Unknown