Cloning and expression of antiviral/ribosome-inactivating protein from Bougainvillea xbuttiana

Journal of biosciences

Harish C Kapoor¹  Nandlal Choudhary¹  Madan L Lodha1 2¹ 

[1] Division of Biochemistry, Indian Agricultural Research Institute, New Delhi 110012, India$$

关键词: Antiviral protein;    BBAP1;    Bougainvillea xbuttiana;    cDNA cloning and expression;    N-glycosidase activity;    ribosome-inactivating;   

DOI  :  

来源: Indian Academy of Sciences

【 摘 要 】

A full-length cDNA encoding ribosome-inactivating/antiviral protein (RIP/AVP) from the leaves of Bougainvillea xbuttiana was isolated. The cDNA consisted of 1364 nucleotides with an open reading frame (ORF) of 960 nucleotides encoding a 35.49 kDa protein of 319 amino acids. The deduced amino acid sequence has a putative active domain conserved in RIPs/AVPs and shows a varying phylogenetic relationship to the RIPs from other plant species. The deduced protein has been designated BBAP1 (Bougainvillea xbuttiana antiviral protein1). The ORF was cloned into an expression vector and expressed in E. coli as a fusion protein of ∼78 kDa. The cleaved and purified recombinant BBAP1 exhibited ribosome-inhibiting rRNA 𝑁-glycosidase activity, and imparted a high level of resistance against the tobacco mosaic virus (TMV).

【 授权许可】

Unknown