Defence transcriptome profiling of Zingiber zerumbet (L.) Smith by mRNA differential display

Journal of biosciences

George Thomas1¹  P G Kavitha¹ 

[1] Plant Molecular Biology Group, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram 695 014, India$$

关键词: Differential display;    ginger;    Pythium aphanidermatum;    soft rot;    transcript profiling;    Zingiber zerumbet;   

DOI  :  

来源: Indian Academy of Sciences

【 摘 要 】

Soft rot is a serious disease in ginger (Zingiber officinale Roscoe), imposing a considerable economic loss annually in all ginger-producing countries. In this study, mRNA differential display was employed to identify genes whose expression was altered in a soft rot-resistant accession of Zingiber zerumbet (L.) Smith, a wild relative of ginger, in response to Pythium aphanidermatum (Edson) Fitzp., which is the principal causative agent of soft-rot disease in ginger. Analysis using 68 primer combinations identified 70 differentially expressed transcript-derived fragments (TDFs), of which 34 TDFs were selected for further analysis following reverse northern screening. Cloning and sequence characterization of the 34 TDFs yielded a total of 54 distinct clones. Functional categorization of these clones revealed seven categories, of which the defence/stress/signalling group was the largest, with clones homologous to genes known to be actively involved in various pathogenesis-related functions in other plant species. The significance of these genes in relation to the resistance response in Z. zerumbet is discussed. This study has provided a pool of candidate genes for detailed molecular dissection of the defence mechanisms in Z. zerumbet and for accessing wild genetic resources for the transgenic improvement of ginger.

【 授权许可】

Unknown