Potential application of urea-derived herbicides as cytokinins in plant tissue culture

Journal of biosciences

Vasanthi Nachiappan¹  Malathi Srinivasan²  Ram Rajasekharan1² 

[1] Department of Biochemistry, School of Life Sciences, Bharathidasan University, Tiruchirappalli 620 024, India.$$;Department of Biochemistry, Indian Institute of Science, Bangalore 560 012, India$$

关键词: Callusing;    cytokinin-like activity;    Coleus forskohlii;    growth regulator;    herbicides;    edicago sativa;   

DOI  :  

来源: Indian Academy of Sciences

【 摘 要 】

Various urea-derived herbicides and different cytokinin analogues were used to determine their effects on callusing response and shoot regenerating capacity of alfalfa (Medicago sativa L.) and Coleus (Coleus forskohlii Briq.). The herbicides monuron and diuron evoked profuse callusing response from Coleus leaf segments and alfalfa petiole explants on Murashige and Skoog medium. Shoot regeneration by monuron (2.0 mg/l) showed a maximum of 3 multiple shoots both in alfalfa and Coleus with a frequency of 92% and 75%, respectively. Whereas diuron (0.5 mg/l) showed a high frequency of shoot regeneration (89%) with a mean number of 5 shoots in alfalfa, in C. forskohlii, the frequency of regeneration was 90% with a mean number of 6 shoots. Diuron with two chloride groups in the phenyl ring showed significantly higher cytokinin-like activity than single chloride substitution monuron. This study demonstrates the potential use of monuron and diuron as cytokinins in plant tissue culture.

【 授权许可】

Unknown