Isolation and characterization of a new mannose-binding lectin gene from Taxus media

Journal of biosciences

Lingxia Zhao¹  Guoyin Kai¹  Kexuan Tanga1¹  Zhiqi Miao¹  Jingui Zheng²  Lei Zhang³  Xiaofen Sun⁴ 

[1] Plant Biotechnology Research Center, School of Agriculture and Biology, Fudan-SJTU-Nottingham Plant Biotechnology R and D Center, School of Life Sciences and Technology, Shanghai Jiao Tong University, Shanghai 200030, People’s Republic of China$$;Biotechnology Center, Fujian Agricultural and Forest University, Fuzhou 350002, People’s Republic of China$$;School of Pharmacy, Second Military Medical University, Shanghai 200433, People’s Republic of China$$;State Key Laboratory of Genetic Engineering, Fudan-SJTU-Nottingham Plant Biotechnology R and D Center, School of Life Sciences, Morgan-Tan International Center for Life Sciences, Fudan University, Shanghai 200433, People’s Republic of China$$

关键词: Evolution analysis;    mannose-binding lectin;    molecular modelling;    Taxus media;    TMA;   

DOI  :  

来源: Indian Academy of Sciences

【 摘 要 】

In this paper, we report the cloning and characterization of the first mannose-binding lectin gene from a gymnosperm plant species, Taxus media. The full-length cDNA of T. media agglutinin (TMA) consisted of 676 bp and contained a 432 bp open reading frame (ORF) encoding a 144 amino acid protein. Comparative analysis showed that TMA had high homology with many previously reported plant mannose-binding lectins and that tma encoded a precursor lectin with a 26-aa signal peptide. Molecular modelling revealed that TMA was a new mannose-binding lectin with three typical mannose-binding boxes like lectins from species of angiosperms. Tissue expression pattern analyses revealed that tma is expressed in a tissue-specific manner in leaves and stems, but not in fruits and roots. Phylogenetic tree analyses showed that TMA belonged to the structurally and evolutionarily closely related monocot mannose-binding lectin superfamily. This study provides useful information to understand the molecular evolution of plant lectins.

【 授权许可】

Unknown