mRNA translation during oocyte maturation plays a key role in development of primordial germ cells in Xenopus embryos

Journal of biosciences

Bahman Zeynali1¹  Keith E Dixon² 

[1] Department of Biology, Faculty of Science, University of Tehran, Tehran, Iran$$;Flinders University of South Australia, Bedford Park 5042, SA, Australia$$

关键词: Chloramphenicol;    primordial germ cells (PGCs);    Xenopus embryos;   

DOI  :  

来源: Indian Academy of Sciences

【 摘 要 】

It is believed that cytoplasmic localization in the egg is necessary for development of primordial germ cells (PGCs) in Xenopus embryos. In this study, we sought to determine if translation of maternal mRNA during oocyte maturation is involved in the development of PGCs. Donor oocytes were collected from both stimulated (those who receive gonadotropin) and unstimulated females, artificially matured and fertilized using a host transfer technique. Using chloramphenicol (50 𝜇M and 500 𝜇M RNA), RNA translation was inhibited during oocyte maturation. Our results showed that in unstimulated embryos treated with 50 𝜇M chloramphenicol, there was a significant reduction in the number of PGCs reaching genital ridges. In stimulated embryos, however, the number of PGCs was unchanged unless a higher concentration (500 𝜇M) of chloramphenicol was used. From these results it is suggested that maternal mRNA translation during oocyte maturation plays a key role in development of PGCs.

【 授权许可】

Unknown