Involvement of Leishmania donovani major surface glycoprotein gp63 in promastigote multiplication

Journal of biosciences

Sanjeev Pandey¹  Dwijen Sarkar¹  Phuljhuri Chakraborti¹  Santu Bandyopadhyay¹  Rakhi Sharma¹  Samit Adhya1¹ 

[1] Indian Institute of Chemical Biology, 4 Raja SC Mullick Road, Kolkata 700 032, India$$

关键词: Antisense;    cytokinesis;    gp63;    Leishmania;   

DOI  :  

来源: Indian Academy of Sciences

【 摘 要 】

The major surface glycoprotein gp63 of the kinetoplastid protozoal parasite Leishmania is implicated as a ligand mediating uptake of the parasite into, and survival within, the host macrophage. By expressing gp63 antisense RNA from an episomal vector in L. donovani promastigotes, gp63-deficient transfectants were obtained. Reduction of the gp63 level resulted in increased generation times, altered cell morphology, accumulation of cells in the G2/M phase of the cell cycle, and increased numbers of binucleate cells with one or two kinetoplasts. Growth was stimulated, and the number of binucleate cells reduced, by addition to the culture of a bacterially expressed fusion protein containing the fibronectin-like SRYD motif and the zinc-binding (metalloprotease) domain of gp63. These observations support an additional role of gp63 in promastigote multiplication; the fibronectin-like properties of gp63 may be important in this process.

【 授权许可】

Unknown