Hypervariable spacer regions are good sites for developing specific PCR-RFLP markers and PCR primers for screening actinorhizal symbionts

Journal of biosciences

Rajani Varghese¹  Vineeta S Chauhan¹  Arvind K Misra¹ 

[1] Department of Botany, North-Eastern Hill University, Shillong 793 022, India$$

关键词: Actinorhizal association;    Alnus;    Frankia;    molecular marker;    rrn ITS;   

DOI  :  

来源: Indian Academy of Sciences

【 摘 要 】

While the ribosomal RNA like highly conserved genes are good molecular chronometers for establishing phylogenetic relationships, they can also be useful in securing the amplification of adjoining hyper-variable regions. These regions can then be used for developing specific PCR primers or PCR-RFL profiles to be used as molecular markers. We report here the use of ITS region of rrn operon of Frankia for developing PCR-RFL profiles capable of discriminating between closely related frankiae. We have also made use of the ITS1 region of the nuclear rrn operon of Alnus nepalensis (D Don) for designing a PCR primer for specific amplification of nuclear DNA of this tree.

【 授权许可】

Unknown