Neutrophil superoxide-anion generating capacity in chronic smoking: effect of long-term 𝛼-tocopherol therapy

Journal of biosciences

Frouwkje De Waart1¹  Anton F H Stalenhoef¹  Jacqueline De Graaf¹  Heidi L M Hak-Lemmers¹  Lambertus J Hvan Tits¹  Pierre N M Demacker¹ 

[1] Department of Medicine, Division of General Internal Medicine, University Medical Center Nijmegen, and Division of Human Nutrition and Epidemiology, Wageningen University Research Center, The Netherlands$$

关键词: Alpha-tocopherol;    neutrophil;    smoking;    superoxide;   

DOI  :  

来源: Indian Academy of Sciences

【 摘 要 】

We investigated whether long-term 𝛼-tocopherol therapy in chronic smoking affects superoxide generating capacity of neutrophils ex vivo. To this purpose, we randomly assigned 128 male chronic smokers (37 ± 21 pack years of smoking) to treatment with placebo (𝑛 = 64) or 𝛼-tocopherol (400 IU dL-𝛼-tocopherol daily, 𝑛 = 64). After two years of therapy, we measured phorbol 12-myristate 13-acetate-induced superoxide production of isolated neutrophils and of diluted whole blood by monitoring reduction of ferricytochrome 𝑐 and luminolenhanced peroxidase-catalyzed chemiluminescence. Plasma lipids and lipoproteins were not different between the two treatment groups. As expected, concentrations of 𝛼-tocopherol in plasma and in low-density lipoproteins were markedly elevated in the supplemented group compared to the placebo group (+ 120%, P < 0.0001 and + 83%, 𝑃 < 0.0001, respectively). Consequently, resistance to in vitro oxidation of low-density lipoproteins (reflected by lag time of conjugated diene formation) was higher in the supplemented group than in the placebo group (+ 22%, 𝑃 < 0.0001). Superoxide generating capacity of neutrophils and superoxide production in diluted whole blood did not differ between 𝛼-tocopherol and placebo group. It is concluded that in chronic smoking long-term supranormal 𝛼-tocopherol intake does not reduce neutrophil superoxide-anion generating capacity, despite large increases in the concentrations of 𝛼-tocopherol in plasma and in low-density lipoproteins.

【 授权许可】

Unknown