Investigating amoebic pathogenesis using Entamoeba histolytica DNA microarrays

Journal of biosciences

Upinder Singh1¹  Preetam Shah¹ 

[1] Departments of Internal Medicine and Microbiology, Stanford University, Stanford, CA 94305, USA$$

关键词: Departments of Internal Medicine and Microbiology;    Stanford University;    Stanford;    CA 94305;    USA;   

DOI  :  

来源: Indian Academy of Sciences

【 摘 要 】

Entamoeba histolytica, a protozoan parasite, causes diarrhea and liver abscesses resulting in 50 million cases of infection worldwide annually. Elucidation of parasite virulence determinants has recently been investigated using genetic approaches. We have undertaken a genomics approach to identify novel virulence determinants in the parasite. A DNA microarray of E. histolytica is being developed based on sequenced genomic clones from the genome sequencing efforts of The Institute of Genomic Research (TIGR) and the Sanger Center. Hybridization of the slides with samples labelled differentially using fluorescent dyes allows the characterization of transcriptional profiles of genes under the biological conditions tested. Additionally, a genome-wide comparison of E. histolytica and E. dispar can be undertaken. The development of an E. histolytica microarray will be outlined and its uses in identifying novel virulence determinants and characterizing amoebic biology will be discussed.

【 授权许可】

Unknown