Amoebapore is an important virulence factor of Entamoeba histolytica

Journal of biosciences

Rivka Bracha¹  David Mirelman1¹  Yael Nuchamowitz¹ 

[1] Department of Biological Chemistry, Weizmann Institute of Science, Rehovot 76100, Israel$$

关键词: Amoebapore;    Entamoeba histolytica;    virulence factor;   

DOI  :  

来源: Indian Academy of Sciences

【 摘 要 】

We have previously demonstrated that inhibition of expression of amoebapore A (AP-A) by antisense RNA caused a marked decrease in the virulence of the parasite. A four-fold over-expression of AP-A was obtained with plasmid (pA7) which has the ap-a gene under the control of gene EhgLE-3-RP-L21. The virulence of the transfected trophozoites, however, was also decreased. Excess of AP-A protein was found in the cytosol and a significant amount was released into the surrounding media. Transfection of the parasite with a plasmid (psAP-1) in which the ap-a gene was introduced with its own regulatory sequences, caused a total suppression of the transcription and translation of both the genomic and episomal ap-a genes. The silenced transfectant was not virulent at all. These results demonstrate that important factors need to be expressed at the correct cellular location and that the parasite has additional internal control mechanisms such as transcriptional gene silencing which can prevent excess amounts of gene expression.

【 授权许可】

Unknown