Journal of biosciences | |
Tyrosine phosphorylation of the human guanylyl cyclase C receptor | |
K Vijayachandra3  Roy Mathew2  Rashna Bhandari1  Sandhya S Visweswariah11  | |
[1] Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bangalore 560 012, India$$;Present address: Department of Animal and Avian Sciences, University of Maryland at College Park, MD 20742, USA$$;Laboratory of Cellular Carcinogenesis and Tumor Promotion, National Cancer Institute, National Institute of Health, Bethesda, MD 20892, USA$$ | |
关键词: EphB1; guanylyl cyclase C; tyrosine phosphorylation; | |
DOI : | |
来源: Indian Academy of Sciences | |
【 摘 要 】
Tyrosine phosphorylation events are key components of several cellular signal transduction pathways. This study describes a novel method for identification of substrates for tyrosine kinases. Co-expression of the tyrosine kinase EphB1 with the intracellular domain of guanylyl cyclase C (GCC) in Escherichia coli cells resulted in tyrosine phosphorylation of GCC, indicating that GCC is a potential substrate for tyrosine kinases. Indeed, GCC expressed in mammalian cells is tyrosine phosphorylated, suggesting that tyrosine phosphorylation may play a role in regulation of GCC signalling. This is the first demonstration of tyrosine phosphorylation of any member of the family of membrane-associated guanylyl cyclases.
【 授权许可】
Unknown
【 预 览 】
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RO201912040494031ZK.pdf | 2308KB | download |