Tyrosine phosphorylation of the human guanylyl cyclase C receptor

Journal of biosciences

K Vijayachandra³  Roy Mathew²  Rashna Bhandari¹  Sandhya S Visweswariah1¹ 

[1] Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bangalore 560 012, India$$;Present address: Department of Animal and Avian Sciences, University of Maryland at College Park, MD 20742, USA$$;Laboratory of Cellular Carcinogenesis and Tumor Promotion, National Cancer Institute, National Institute of Health, Bethesda, MD 20892, USA$$

关键词: EphB1;    guanylyl cyclase C;    tyrosine phosphorylation;   

DOI  :  

来源: Indian Academy of Sciences

【 摘 要 】

Tyrosine phosphorylation events are key components of several cellular signal transduction pathways. This study describes a novel method for identification of substrates for tyrosine kinases. Co-expression of the tyrosine kinase EphB1 with the intracellular domain of guanylyl cyclase C (GCC) in Escherichia coli cells resulted in tyrosine phosphorylation of GCC, indicating that GCC is a potential substrate for tyrosine kinases. Indeed, GCC expressed in mammalian cells is tyrosine phosphorylated, suggesting that tyrosine phosphorylation may play a role in regulation of GCC signalling. This is the first demonstration of tyrosine phosphorylation of any member of the family of membrane-associated guanylyl cyclases.

【 授权许可】

Unknown