Avirulent mutants of Macrophomina phaseolina and Aspergillus fumigatus initiate infection in Phaseolus mungo in the presence of phaseo-linone; levamisole gives protection

Journal of biosciences

Santosh K Mishra¹  Kazia I Siddiqui1¹  Suchandra Sett¹ 

[1] Indian Institute of Chemical Biology, 4, Raja S C Mullick Road, Jadavpur, Calcutta 700 032, India$$

关键词: Aspergillus;    immunopotentiator;    infection;    levamisole;    macrophomina;    phaseolinone;    protection;   

DOI  :  

来源: Indian Academy of Sciences

【 摘 要 】

To evaluate the role of phaseolinone, a phytotoxin produced by Macrophomina phaseolina, in disease initiation, three nontoxigenic avirulent mutants of the fungus were generated by UV-mutagenesis. Two of them were able to initiate infection in germinating Phaseolus mungo seeds only in the presence of phaseolinone. The minimum dose of phaseolinone required for infection in 30% seedlings was 2·5 𝜇g/ml. A human pathogen, Aspergillus fumigatus was also able to infect germinating seeds of P. mungo in the presence of 5 𝜇g/ml concentration of phaseolinone. Phaseolinone seemed to facilitate infection by A. fumigatus, which is not normally phytopathogenic, by reducing the immunity of germinating seedlings in a nonspecific way. Levamisole, a non-specific immunopotentiator gave protection against infection induced by A. fumigatus at an optimum dose of 50 𝜇g/ml. Sodium malonate prevented the effects of levamisole.

【 授权许可】

Unknown