Different responses of soybean cyst nematode resistance between two RIL populations derived from Peking × 7605 under two ecological sites

Journal of genetics

BIN ZHOU¹  JUNYI GAI¹  HUI DING¹  NA GUO¹  YONGCHUN LI¹  HAN XING1¹  RAN XU²  WEIGUO ZHAO³  JINMING ZHAO¹ 

[1] State Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, Jiangsu 210095, People’s Republic of China$$;Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan, Shandong 250100, People’s Republic of China$$;Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang, Jiangsu 212018, People’s Republic of China$$

关键词: soybean cyst nematode;    resistance;    inheritance;    simple sequence repeat marker.;   

DOI  :  

学科分类：生物科学（综合）

来源: Indian Academy of Sciences

【 摘 要 】

The soybean cyst nematode (SCN), Heterodera glycines Ichinohe, is a plant-parasitic nematode that feeds on the roots of soybean and most economically devastating pathogen of soybean (Glycine max (L.) Merr.) worldwide. Host plant resistance is the most effective control method. To understand SCN resistance in different environments, two recombinant-inbred lines (RILs) populations NJ(RN)P7 (217 F2:8:11 lines) and JN(RN)P7 (248 F2:7:9 lines) were developed from the cross of the cultivars Peking × 7605 in Nanjing and Jinan, respectively, and examined in this study. Peking is resistant to SCN race 1 (HG types 2.5.7), while 7605 is highly susceptible. Chi-square test of frequency distribution of families’ female index (FI) showed that resistance to SCN was significantly different between NJ(RN)P7 and JN(RN)P7 populations. Three recessive genes conditioned the inheritance of resistance to SCN race 1 in both populations, but significant difference was detected for the mean of FI on two populations (DM= −16.68, P < 0.01). This indicated that natural selection may affect resistance to SCN. By analysing the variation of phenotype, the genetic structure of the two populations was determined to be different. The inheritance and variation of resistance were confirmed by simple sequence repeat (SSR) markers. For the two populations, 10 SSR markers showed polymorphism of resistant and susceptible DNA bulks. Some markers associated with the resistance of SCN races 1, 2, 3 and 5, and two markers, Satt163 and Satt309, reportedly related to rgh1 were detected both in NJ(RN)P7 and JN(RN)P7 populations. The results support the view that a disease acts as a selective force on plant resistance characteristics, which may alter the relative fitness of resistance alleles.

【 授权许可】

Unknown