A substitution mutation in OsCCD7 cosegregates with dwarf and increased tillering phenotype in rice

Journal of genetics

Trilochan Mohapatra³  Krishnanand P. Kulkarni³  Chandrapal Vishwakarma³  M. Seshashayee¹  Nagendra K. Singh³  Kuldeep Singh⁵  N. Sarla²  Manoj Nath³  R. P. Sharma1³  John M. Lima³  S. Robin⁴  Prasad Dokku³  Rajesh N. Gacche⁶  Sarada P. Sahoo³  Ashok K. Singh⁷ 

[1] University of Agricultural Sciences, GKVK, Bangalore 560 065, India$$;Directorate of Rice Research, Rajendranagar, Hyderabad 500 030, India$$;National Research Centre on Plant Biotechnology, LBS Centre, Indian Agricultural Research Institute, Pusa Campus, New Delhi 110 012, India$$;Tamilnadu Agricultural University, Coimbatore 641 003, India$$;Punjab Agricultural University, Ludhiana 141 027, India$$;School of Life Sciences, Swami Ramanand Teerth Marathwada University, Nanded 431 606, India$$;Indian Agricultural Research Institute, Pusa Campus, New Delhi 110 012, India$$

关键词: rice;    dwarf;    tillering;    mutant;    QTL;    CCD7.;   

DOI  :  

学科分类：生物科学（综合）

来源: Indian Academy of Sciences

【 摘 要 】

Dwarf plant height and tillering ability are two of the most important agronomic traits that determine the plant architecture, and have profound influence on grain yield in rice. To understand the molecular mechanism controlling these two traits, an EMS-induced recessive dwarf and increased tillering1 (dit1) mutant was characterized. The mutant showed proportionate reduction in each internode as compared to wild type revealing that it belonged to the category of dn-type of dwarf mutants. Besides, exogenous application of GA3 and 24-epibrassinolide, did not have any effect on the phenotype of the mutant. The gene was mapped on the long arm of chromosome 4, identified through positional candidate approach and verified by cosegregation analysis. It was found to encode carotenoid cleavage dioxygenase7 (CCD7) and identified as an allele of htd1. The mutant carried substitution of two nucleotides CC to AA in the sixth exon of the gene that resulted in substitution of serine by a stop codon in the mutant, and thus formation of a truncated protein, unlike amino acid substitution event in htd1. The new allele will facilitate further functional characterization of this gene, which may lead to unfolding of newer signalling pathways involving plant development and architecture.

【 授权许可】

Unknown