Roles of the troponin isoforms during indirect flight muscle development in Drosophila

Journal of genetics

Salam Herojeet Singh²  Upendra Nongthomba1²  Prabodh Kumar²  Nallur B. Ramachandra2¹ 

[1] Department of Studies in Zoology, University of Mysore, Manasagangotri, Mysore 570 006, India$$;Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bangalore 560 012, India$$

关键词: troponin;    isoforms;    myofibril;    flight muscle;    Drosophila.;   

DOI  :  

学科分类：生物科学（综合）

来源: Indian Academy of Sciences

【 摘 要 】

Troponin proteins in cooperative interaction with tropomyosin are responsible for controlling the contraction of the striated muscles in response to changes in the intracellular calcium concentration. Contractility of the muscle is determined by the constituent protein isoforms, and the isoforms can switch over from one form to another depending on physiological demands and pathological conditions. In Drosophila, amajority of themyofibrillar proteins in the indirect flight muscles (IFMs) undergo post-transcriptional and post-translational isoform changes during pupal to adult metamorphosis to meet the high energy and mechanical demands of flight. Using a newly generated Gal4 strain (UH3-Gal4) which is expressed exclusively in the IFMs, during later stages of development, we have looked at the developmental and functional importance of each of the troponin subunits (troponin-I, troponin-T and troponin-C) and their isoforms. We show that all the troponin subunits are required for normal myofibril assembly and flight, except for the troponin-C isoform 1 (TnC1). Moreover, rescue experiments conducted with troponin-I embryonic isoform in the IFMs, where flies were rendered flightless, show developmental and functional differences of TnI isoforms and importance of maintaining the right isoform.

【 授权许可】

Unknown