Intraspecific chromosomal and genetic polymorphism in Brassica napus L. detected by cytogenetic and molecular markers

Journal of genetics

Alexandr V. Zelenin¹  Galina V. Mozgova⁴  Lyudmila V. Zemtsova¹  Valentina A. Lemesh⁴  Yadviga E. Pilyuk²  Zoya E. Grushetskaya⁴  Valentina T. Volovik³  Alexandra V. Amosova1¹  Olga V. Muravenko¹  Tatiana E. Samatadze¹  Natalia V. Melnikova¹ 

[1]Engelgardt Institute of Molecular Biology of RAS, Vavilov Str. 32, Moscow 119991, Russia$$

[2]Scientific and Practical Centre of NAS of Belarus for Arable Farming, Timiryazev Str. 1, Zhodino 222160, Belarus$$

[3]All-Russian Williams Fodder Research Institute of RAAS, Lobnya Moscow region 141005, Russia$$

[4]Institute of Genetics and Cytology of NAS of Belarus, Academichnaya Str. 27, Minsk 220072, Belarus$$

关键词: C-banding;    fluorescence in situ hybridization;    FAE1.1 gene;    idiogram;    rDNA sites;    Brassica napus.;   

DOI  :  

学科分类：生物科学（综合）

来源: Indian Academy of Sciences

【 摘 要 】

The application of DNA intercalator 9-aminoacridine allowed us to increase the resolution of chromosome C-banding and DAPI-banding patterns and to investigate chromosomal polymorphism in karyotypes of seven spring and six winter rape varieties. It was shown that the pericentromeric and intercalary C-bands of most of the chromosomes in spring rape were smaller in size and less polymorphic than those of winter rape. More 26S and 5S rDNA sites were found in the winter rape karyotypes than the spring varieties. Separate or colocalized 26S and 5S rDNA sites were revealed on chromosomes 4, 5, 6, 8, 10, 14, 15, 16 and 18. Intervarietal and intravarietal polymorphism of the number and chromosomal localization of rDNA sites were detected. The generalized idiogram of chromosomes of 13 Brassica napus varieties with account of all possibilities of C-banding patterns as well as localization of 26S and 5S rDNA sites were constructed. Polymorphism of the examined molecular and cytogenetic markers as well as the heterozygosis level of FAE1.1 gene controlling erucic acid synthesis in rapeseed was higher in the winter varieties than in the spring ones. The obtained data were in a satisfactory agreement with increased tolerance to environmental stress conditions of winter rape.

【 授权许可】

Unknown