Expression profile of genes coding for carotenoid biosynthetic pathway during ripening and their association with accumulation of lycopene in tomato fruits

Journal of genetics

Shuchi Smita²  Sangram Keshari Lenka²  Amit Katiyar²  Ravi Rajwanshi²  Viswanathan Chinnusamy¹  Kailash Chander Bansal1 7² 

[1]Division of Plant Physiology, Indian Agricultural Research Institute Campus, New Delhi 110 012, India$$

[2]National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute Campus, New Delhi 110 012, India$$

关键词: carotenoid biosynthesis;    carotenoid pathway genes;    tomato;    lycopene;    Pusa Rohini;    ripening.;   

DOI  :  

学科分类：生物科学（综合）

来源: Indian Academy of Sciences

【 摘 要 】

Fruit ripening process is associated with change in carotenoid profile and accumulation of lycopene in tomato (Solanum lycopersicum L.). In this study, we quantified the 𝛽-carotene and lycopene content at green, breaker and red-ripe stages of fruit ripening in eight tomato genotypes by using high-performance liquid chromatography. Among the genotypes, lycopene content was found highest in Pusa Rohini and lowest in VRT-32-1. To gain further insight into the regulation of lycopene biosynthesis and accumulation during fruit ripening, expression analysis of nine carotenoid pathway-related genes was carried out in the fruits of high lycopene genotype—Pusa Rohini. We found that expression of phytoene synthase and 𝛽-carotene hydroxylase-1 was four and thirty-fold higher, respectively, at breaker stage as compared to red-ripe stage of fruit ripening. Changes in the expression level of these genes were associated with a 40% increase in lycopene content at red-ripe stage as compared with breaker stage. Thus, the results from our study suggest the role of specific carotenoid pathway-related genes in accumulation of high lycopene during the fruit ripening processes.

【 授权许可】

Unknown