Marker-assisted selection of highmolecular weight glutenin alleles related to bread-making quality in Iranian common wheat (Triticum aestivum L.)

Journal of genetics

Bahman Yazdi-Samadi¹  Ali Izadi-Darbandi1² 

[1]Department of Agronomy and Plant Breeding, College of Agricultural and Natural Resources, University of Tehran, P. O. Box 31587-77871, Karaj, Iran$$

[2]Department of Agronomy and Plant Breeding sciences, College of Aburaihan, University of Tehran, P. O. Box 3391653775, Pakdasht, Iran$$

关键词: wheat;    bread-making;    HMW-GS;    DNA marker.;   

DOI  :  

学科分类：生物科学（综合）

来源: Indian Academy of Sciences

【 摘 要 】

Bread-making quality in hexaploid wheats is a complex trait. It has been shown that the amount and composition of protein can influence dough rheological properties. The high-molecular-weight (HMW) glutenins are encoded by a complex locus, Glu-1, on the long arm of group-1 homoeologus chromosome of the A, B and D genomes. In this work we used PCR-based DNA markers as a substitution tool to distinguish wheat bread-making quality. We detected PCR-based DNA markers for coding sequence of Glu-A1x, Glu-B1x and Glu-D1x to be 2300 bp, 2400 bp and 2500 bp respectively. DNA markers related to coding sequence of Glu-A1y, Glu-B1y and Glu-D1y were; 1800 bp, 2100 bp and 1950 bp, however, the repetitive region of their coding sequence were shown to be about 1300 bp, 1500 bp and 1600 bp. The results demonstrate that the size variation was due to different lengths of the central repetitive domain. Good or poor bread-making quality in wheat is associated with two allelic pairs of Glu-D1, designated 1Dx5-1Dy10 and IDx2-1Dy12. The 1Bx7 allele has moderate-to-good quality score. The specific DNA markers, of 450 bp, 576 bp, 612 bp and 2400 bp respectively were characterized for 1Dx5, 1Dy10, 1Dy12 and 1Bx7 alleles. These markers are very important in screening of wheat for bread-making quality.

【 授权许可】

Unknown