Genomic dissection and prioritizing of candidate genes of QTL for regulating spontaneous arthritis on chromosome 1 in mice deficient for interleukin-1 receptor antagonist

Journal of genetics

John M. Stuart¹  Jifei Zhang²  Feng Jiao⁴  Robert W. Williams³  Yan Jiao⁴  Weikuan Gu2⁴  Jian Yan¹  Karen A. Hasty⁴  Yanhong Cao⁵  Xiaoyun Liu⁴ 

[1] Department of Medicine, University of Tennessee Health Science Center, Memphis, TN 38163, USA$$;Mudanjiang Medical College, Mudanjiang, 157001, People’s Republic of China$$;Department of Anatomy and Neurobiology, University of Tennessee Health Science Center, Memphis, TN 38163, USA$$;Department of Orthopaedic Surgery, Campbell Clinic and Pathology, University of Tennessee Health Science Center, Memphis, TN 38163, USA$$;Institute of Kaschin-Beck Disease, Center for Endemic Disease Control, Centers for Disease Control and Prevention, Harbin Medical University, Harbin, 150081, People’s Republic of China$$

关键词: arthritis;    interleukin-1 receptor antagonist;    microarray;    mouse;    QTL;    SNP.;   

DOI  :  

学科分类：生物科学（综合）

来源: Indian Academy of Sciences

【 摘 要 】

Rheumatoid arthritis is a heterogeneous disease with clinical and biological polymorphisms. IL-1RN is a protein that binds to interleukin-1 (IL-1) receptors and inhibits the binding of IL-1-alpha and IL-1-beta. IL-1RN levels are elevated in the blood of patients with a variety of infectious, immune, and traumatic conditions. Balb/c mice deficient in IL-1ra (mouse gene of IL-1RN) develop spontaneous autoimmune arthritis while DBA/1 mice deficient in IL-1ra do not. Previously, we identified a major QTL that regulates the susceptibility to arthritis in Balb/c mice with IL-1ra deficiency. In this study, we found that the QTL may contain two peaks that are regulated by two sets of candidate genes. By haplotype analysis, the total genomic regions of candidate genes were reduced from about 19 Mbp to approximately 9 Mbp. The total number of candidate genes was reduced from 208 to 21.

【 授权许可】

Unknown