Mutations in the 𝑆 gene region of hepatitis B virus genotype 𝐷 in Turkish patients

Journal of genetics

Ersan Özaslan2³  Arzu Barsgan²  Mehmet Koruk¹  Mehmet Özaslan² 

[1] Department of Gastroenterology, University of Gaziantep, 27310/ Gaziantep, Turkey$$;Department of Biology, University of Gaziantep, 27310/ Gaziantep, Turkey$$;Department of Gastroenterology, Ankara Numune Education and Training Hospital, 06100 / Ankara, Turkey$$

关键词: Hepatitis B virus (HBV);    hepatitis B surface antigen;    genotype 𝐷;    𝑆 gene region;    mutation.;   

DOI  :  

学科分类：生物科学（综合）

来源: Indian Academy of Sciences

【 摘 要 】

The 𝑆 gene region of the hepatitis B virus (HBV) is responsible for the expression of surface antigens and includes the ‘a’-determinant region. Thus, mutation(s) in this region would afford HBV variants a distinct survival advantage, permitting the mutant virus to escape from the immune system. The aim of this study was to search for mutations of the 𝑆 gene region in different patient groups infected with genotype 𝐷 variants of HBV, and to analyse the biological significance of these mutations. Moreover, we investigated 𝑆 gene mutation inductance among family members. Forty HBV-DNA-positive patients were determined among 132 hepatitis B surface antigen (HbsAg) carriers by the first stage of seminested PCR. Genotypes and subtypes were established by sequencing of the amplified S gene regions. Variants were compared with original sequences of these serotypes, and mutations were identified. All variants were designated as genotype 𝐷 and subtype ayw3. Ten kinds of point mutations were identified within the 𝑆 region. The highest rates of mutation were found in chronic hepatitis patients and their family members. The amino acid mutations 125 (M → T) and 127 (T → P) were found on the first loop of ‘a’-determinant. The other consequence was mutation inductance in a family member. We found some mutations in the S gene region known to be stable and observed that some of these mutations affected 𝑆 gene expression.

【 授权许可】

Unknown