The inactive X chromosome in the human female is enriched in 5-methylcytosine to an unusual degree and appears to contain more of this modified nucleotide than the remainder of the genome

Journal of genetics

H. Sharat Chandra3 2¹  Deepti D. Deobagkar1 2² 

[1] Centre for Human Genetics, G-04, ITPL, Whitefield, Bangalore 560 066, India$$;Department of Zoology, University of Pune, Pune 411 007, India$$

关键词: 5-methylcytosine;    X inactivation;    human X chromosome;    methylation;    photoacoustic spectroscopy.;   

DOI  :  

学科分类：生物科学（综合）

来源: Indian Academy of Sciences

【 摘 要 】

By employing a procedure that combines ELISA and photoacoustic spectroscopy, we have examined the content of 5-methylcytosine (m5C) in DNA of individuals who differed from one another in the number of X chromosomes in their genomes. The results show that the human inactive X chromosome (Xi) contains very high amounts of this modified nucleotide. We estimate that in the 46,XX female there is more m5C in Xi (∼3.6 × 107) than in all the remaining chromosomes put together (∼2.1 × 107). Our results also suggest that nearly one-fifth of all cytosines in Xi are methylated and that, in addition to CpG methylation, there is extensive non-CpG methylation as well.

【 授权许可】

Unknown