Identification of four genes involved in suppression of the pre-mRNA splicing defect in the sng1-1/rhp6- mutant of fission yeast

Journal of genetics

Jagmohan Singh1¹  Alpana Naresh¹ 

[1] Institute of Microbial Technology, Sector 39A, Chandigarh 160 036, India$$

关键词: pre-mRNA splicing;    suppressors;    Schizosaccharomyces pombe.;   

DOI  :  

学科分类：生物科学（综合）

来源: Indian Academy of Sciences

【 摘 要 】

Apart from the global regulators of silencing in the fission yeast Schizosaccharomyces pombe, namely swi6, clr1, clr2, clr3, clr4 and rik1, the DNA repair gene rhp6 plays a unique role in mating-type silencing. Recently, we showed that sng1-1, a mutation in the 5′ splice junction of the second intron of the rhp6 gene, leads to derepression of both the silent loci mat2 and mat3 in switching background. To address the mechanism of rhp6 in silencing, we have isolated several extragenic suppressors of the sng1-1/rhp6- mutation. These suppressors fall into four complementation groups and are referred to as suppressor of rhp6: sur1, sur2, sur3 and sur4. Interestingly, reverse transcriptase polymerase chain reaction analysis of the rhp6 transcript shows that in contrast to about > 50% level of unspliced rhp6 pre-mRNA in the sng1-1/rhp6- mutant, there is a restoration of normal splicing to varying degrees in the suppressors. The sur2 gene belongs to the AAA-ATPase family of proteins, with maximum homology to the SIN1-associated protein SAP1 of Saccharomyces cerevisiae. We propose that sur2, along with sur1, sur3 and sur4, may play an as yet uncharacterized role in pre-mRNA splicing.

【 授权许可】

Unknown