Lack of functional P2X7 receptor aggravates brain edema development after middle cerebral artery occlusion

Purinergic Signalling

Wolfgang Nörenberg¹  Melanie Kaiser¹  Daniel Huster¹  Anja Penk¹  Michael Schaefer¹  Ute Krügel¹  Heike Franke¹ 

[1] Universität Leipzig$$

关键词: Purinergic receptor;   

DOI  :  10.1007/s11302-016-9511-x

学科分类：分子生物学，细胞生物学和基因

来源: Springer

【 摘 要 】

Effective therapeutic measures against the development of brain edema, a life-threatening complication of cerebral ischemia, are necessary to improve the functional outcome for the patient. Here, we identified a beneficial role of purinergic receptor P2X7 activation in acute ischemic stroke. Involvement of P2X7 in the development of neurological deficits, infarct size, brain edema, and glial responses after ischemic cerebral infarction has been analyzed. Neurologic evaluation, magnetic resonance imaging, and immunofluorescence assays were used to characterize the receptor’s effect on the disease progress during 72 h after transient middle cerebral artery occlusion (tMCAO). Sham-operated animals were included in all experiments for control purposes. We found P2X7-deficient mice to develop a more prominent brain edema with a trend towards more severe neurological deficits 24 h after tMCAO. Infarct sizes, T2 times, and apparent diffusion coefficients did not differ significantly between wild-type and P2X7^−/− animals. Our results show a characteristic spatial distribution of reactive glia cells with strongly attenuated microglia activation in P2X7^−/− mice 72 h after tMCAO. Our data indicate that P2X7 exerts a role in limiting the early edema formation, possibly by modulating glial responses, and supports later microglia activation.

【 授权许可】

Unknown