Ecto-nucleotidases distribution in human cyclic and postmenopausic endometrium

Purinergic Signalling

Inmaculada Gómez de Aranda³  Enric Condom⁵  Jean Sévigny¹  Jordi Ponce⁴  Mireia Martín-Satué²  Benjamín Torrejón-Escribano⁸  August Vidal⁵  Maria del Rosario Taco⁶  Elisabet Aliagas⁷ 

[1] Université Laval$$;Universitat de Barcelona$$Institut d’Investigació Biomèdica de Bellvitge (IDIBELL)$$L’Hospitalet de Llobregat$$;Universitat de Barcelona$$;Institut d’Investigació Biomèdica de Bellvitge (IDIBELL)$$Servei de Ginecologia, Hospital de Bellvitge$$;Universitat de Barcelona$$Institut d’Investigació Biomèdica de Bellvitge (IDIBELL)$$Servei d’Anatomia Patològica, Hospital Universitari de Bellvitge$$;Servei d’Anatomia Patològica, Hospital Universitari de Bellvitge$$;Universitat de Barcelona$$Institut d’Investigació Biomèdica de Bellvitge (IDIBELL)$$;Universitat de Barcelona$$Universitat de Barcelona$$

关键词: NPP;   

DOI  :  10.1007/s11302-012-9345-0

学科分类：分子生物学，细胞生物学和基因

来源: Springer

【 摘 要 】

Extracellular ATP and its hydrolysis product, adenosine, acting through specific receptors collectively named purinergic receptors, regulate female fertility by influencing the endometrial fluid microenvironment. There are four major groups of ecto-nucleotidases that control the levels of extracellular ATP and adenosine and thus their availability at purinergic receptors: ecto-nucleoside triphosphate diphosphohydrolases (E-NTPDases), ecto-nucleotide pyrophosphatase/phospho-diesterases (E-NPPs), ecto-5′-nucleotidase (5′NT), and alkaline phosphatases (APs). The aim of the present work is to characterize the expression and distribution of ecto-nucleotidases in human endometrium along the menstrual cycle and after menopause, to evaluate their potential utility as fertility markers. We examined proliferative, secretory and atrophic endometria from women without endometrial pathology undergoing hysterectomy. We show that the ecto-nucleotidases are mainly present at endometrial epithelia, both luminal and glandular, and that their expression fluctuates along the cycle and also changes after menopause. An important result was identifying NPP3 as a new biological marker of tubal metaplasia. Our results emphasize the relevance of the study of purinergic signaling in human fertility.

【 授权许可】

Unknown