Amino acid variations resulting in functional and nonfunctional zebrafish P2X1 and P2X5.1 receptors

Purinergic Signalling

John Y. Kuwada¹  Sean E. Low¹  Richard I. Hume¹ 

[1] University of Michigan$$

关键词: Myogenesis;   

DOI  :  10.1007/s11302-008-9124-0

学科分类：分子生物学，细胞生物学和基因

来源: Springer

【 摘 要 】

Several zebrafish P2X receptors (zP2X₁, zP2X₂, and zP2X_5.1) have been reported to produce little or no current although their mammalian orthologs produce functional homomeric receptors. We isolated new cDNA clones for these P2X receptors that revealed sequence variations in each. The new variants of zP2X₁ and zP2X_5.1 produced substantial currents when expressed by Xenopus oocytes, however the new variant of zP2X₂ was still nonfunctional. zP2X₂ lacks two lysine residues essential for ATP responsiveness in other P2X receptors; however introduction of these two lysines was insufficient to allow this receptor to function as a homotrimer. We also tested whether P2X signaling is required for myogenesis or synaptic communication at the zebrafish neuromuscular junction. We found that embryonic skeletal muscle expressed only one P2X receptor, P2X_5.1. Antisense knockdown of P2X_5.1 eliminated skeletal muscle responsiveness to ATP but did not prevent myogenesis or behaviors that require functional transmission at the neuromuscular junction.

【 授权许可】

Unknown