P2 nucleotide receptors on C2C12 satellite cells

Purinergic Signalling

Wiktor Banachewicz¹  Patryk Krzemiński¹  Dorota Supłat¹  Paweł Pomorski¹  Jolanta Barańska¹ 

[1] Polish Academy of Sciences$$

关键词: P2X and P2Y receptors;   

DOI  :  10.1007/s11302-005-6311-0

学科分类：分子生物学，细胞生物学和基因

来源: Springer

【 摘 要 】

In developing muscle cells environmental stimuli transmitted by purines binding to the specific receptors are crucial proliferation regulators. C2C12 myoblasts express numerous purinergic receptors representing both main classes: P2X and P2Y. Among P2Y receptors we have found the expression of P2Y₁, P2Y₂, P2Y₄, P2Y₆ and P2Y₁₂ family members while among P2X receptors P2X₄, P2X₅ and P2X₇ were discovered. We have been able to show that activation of those receptors is responsible for ERK class kinase activity, responsible for regulation of cell proliferation pathway. We have also demonstrated that this activity is calcium dependent suggesting Ca²⁺ ions as secondary messenger between receptor and kinase regulatory system. More specifically, we do suspect that in C2C12 myoblasts calcium channels of P2X receptors, particularly P2X₅ play the main role in proliferation regulation. In further development of myoblasts into myotubes, when proliferation is gradually inhibited, the pattern of P2 receptors is changed. This phenomenon is followed by diminishing of the P2Y₂-dependent Ca²⁺ signaling, while the mRNA expression of P2Y₂ receptor reminds still on the high level. Moreover, P2X₂ receptor mRNA, absent in myoblasts appears in myotubes. These data show that differentiation of C2C12 cell line satellite myoblasts is accompanied by changes in P2 receptors expression pattern.

【 授权许可】

Unknown