Inheritance and Allelic Relationships of Resistance Genes for in ‘Corsica’ and ‘Beeson’ Soybean

Crop Science

Shakiba, Ehsan⁴  Brye, Kris⁴  Chen, Pengyin⁴  Shi, Ainong²  Li, Dexiao¹  Dong, Dekun³ 

[1] College of Agriculture, Northwest Univ. of Agriculture and Forestry, Yangling, Shaanxi 712100, P.R. China$$;Syngenta Seeds, 2369 330th St., Slater, IA 50224$$;Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Science, Hangzhou, Zhejiang 310021, P.R. China$$;Dep. of Crop, Soil, and Environmental Sciences, Univ. of Arkansas, Fayetteville, AR 72701$$

关键词: Segmentation;    Bio-cell Organelles;    Fluorescence Microscopy;    Ellipse Detection;   

DOI  :  10.2135/cropsci2012.01.0006

学科分类：农业科学（综合）

来源: Crop Science

【 摘 要 】

Soybean mosaic virus (SMV) is an important viral disease of soybean [Glycine max (L.) Merr.] and causes a drastic reduction of seed yield and quality. Soybean mosaic virus isolates can be classified into one of seven strain groups, G1 through G7. Three loci with SMV resistance alleles Rsv1, Rsv3, and Rsv4 have been reported so far. Each locus contains multiple alleles: nine alleles at Rsv1, three alleles at Rsv3, and two alleles at Rsv4. Soybean cultivars Corsica (PI 559931) and Beeson (PI 548510) exhibit different patterns of response to SMV strains than differential cultivars with known genes. The objective of this study was to investigate the inheritance and allelism of resistance genes in Corsica and Beeson. To examine the inheritance of SMV resistance, Corsica and Beeson were crossed with the susceptible cultivar Essex, and the F2 and F3 progenies were inoculated with a G7 isolate. To identify the allelic relationship of genes in Corsica and Beeson with known SMV resistance loci, they were crossed with PI 96983 (Rsv1), ‘L29’ (Rsv3), and V94-5152 (Rsv4), and their F2 plants and F2:3 lines were inoculated with G7. A serological tissue blotting immunoassay (TBIA) was used to confirm the presence or absence of the virus in the inoculated plants. Molecular markers were used to confirm the phenotypic data. The results showed a monogenic dominant resistance in Corsica, with the resistant gene located at the Rsv1 locus. Beeson also contains a single dominant gene for SMV resistance, but the gene is located at the Rsv4 locus. Both genes in Corsica and Beeson confer a different SMV reaction pattern than other reported alleles at the same loci. Therefore, we propose the new symbols of Rsv1-c for the resistant gene in Corsica and Rsv4-b for the resistant gene in Beeson. The results from the genetic study were confirmed by the molecular markers and TBIA. The information from this study can be used for parent selection by breeders in cross breeding and gene pyramiding.

【 授权许可】

Unknown