The neuromuscular activity of Micrurus pyrrhocryptus venom and its neutralization by commercial and specific coral snake antivenoms

Journal of Venom Research

Maria Alice da Cruz-Höfling¹  Adolfo Rafael de Roodt¹  Léa Rodrigues-Simioni¹  Thiago Magalhães Camargo¹ 

关键词:  Coral snake venom;    neuromuscular blockade;    neurotoxin;    nicotinic receptor;    postsynaptic;   

DOI  :  

学科分类：药理学

来源: Library Publishing Media

【 摘 要 】

The neuromuscular activity of Micrurus pyrrochryptus venom was studied in chick biventer cervicis (BC) and mouse phrenic nerve-diaphragm (PND) preparations. The venom (0.5-50µg/ml) caused irreversible, time- and concentration-dependent blockade, with BC being more sensitive than PND (50% blockade with 10µg/ml in 22±3min and 62±4min, respectively; mean±SEM, n=6; p<0.05). In BC preparations, venom (0.5µg/ml) progressively abolished ACh-induced contractures, whereas contractures to exogenous KCl and muscle twitches in curarized preparations were unaffected. The venom neither altered creatine kinase release (venom: 25.8±1.75IU/l vs control: 24.3±2.2IU/l, n=6, after 120min), nor it caused significant muscle damage (50µg of venom/ml vs control: 3.5±0.8% vs1.1±0.7% for PND; 4.3±1.5% vs 1.2±0.5% for BC, n=5). The venom had low PLA 2 activity. Neurotoxicity was effectively neutralized by commercial Micrurus antivenom and specific antivenom. These findings indicate that M. pyrrhocryptus venom acts postsynaptically on nicotinic receptors, with no significant myotoxicity.

【 授权许可】

Unknown