Approaches Development and Validation of RP-HPLC Method for Estimation of Glimepiride in Rat Plasma-Application to Pharmacokinetic Studies

Eurasian Journal of Analytical Chemistry

Alok S. Tripathi Anil P. Dewani¹  Anil V. Chandewar Papiya Mitra Mazumder¹ 

关键词: : glimepiride;    RP-HPLC;    rat plasma;   

DOI  :  10.12973/ejac.2016.121a

学科分类：分析化学

来源: Eurasian Journal of Analytical Chemistry

【 摘 要 】

A simple and sensitive method was developed for estimation of Glimepiride (GLIM) in rat Plasma by reverse phase high performance liquid chromatography (RP-HPLC). The drug samples were extracted by liquid-liquid extraction with 300 µL of acetonitrile and 5 mL of diethyl ether. Chromatographic separation was achieved on C18 column using methanol: water (85:15 v/v) as mobile phase at a flow rate of 1ml/min and UV detection at 230 nm. The retention time of GLIM was found to be 2.5 min. The developed method was validated for accuracy, precision, linearity and recovery. Linearity studies were found to be acceptable over the range of 100 – 6000 ng/mL. The method was successfully applied for the analysis of rat plasma sample for application in pharmacokinetic study, bioavailability.

【 授权许可】

Unknown