Evaluation of Antioxidant Activity of Plant-parasitic Macrofungus: Phellinus durissimus (Lloyd) Roy

Eurasian Journal of Analytical Chemistry

Mrugesh D. Shukla¹  Rina H. Gokani¹  Devdas D. Santani¹  Hasmukh A. Modi¹  Suman K. Lahiri¹  Mamta B. Shah¹ 

关键词:  Kinetic spectrophotometry;    UV-Visible region;    nitrite ion;    Cresyl violet perchlorate;   

DOI  :  10.12973/ejac.2010.00079a

学科分类：分析化学

来源: Eurasian Journal of Analytical Chemistry

【 摘 要 】

Phellinus durissimus (Lloyd) Roy is a plant parasitic macrofungal drug, which is known to be used in traditional medicinal practices by tribes of South Gujarat. The methanol extract (PdM) was subjected to in vitro chemical assays like, DPPH (1, 1-diphenyl-2-picryl hydrazyl) free radical, superoxide radical, hydroxyl radical, nitric oxide scavenging activity, erythrocyte hemolysis test, β-carotene bleaching test, linoleic acid - ferric thiocynate and lipid peroxidation which were followed by evaluation of ethyl acetate soluble (PdEs) and ethyl acetate insoluble (PdEi) fractions of PdM, by DPPH free radical scavenging activity, linoleic acid-ferric thiocynate method and β-carotene bleaching test. PdM showed considerable antioxidant activity in most of the assays, yielding IC50 values between 50 to 100 μg mL-1, baring hydroxyl radical scavenging activity and erythrocyte hemolysis test of which the activity of the former assay was much lower but the later was higher than the rest of the assays. PdEs and PdEi showed substantial activity but performed best either of the DPPH and lipid peroxidation assays. The current work is the first report that establishes P. durissimus has significant antioxidant potential which is comparable to or better than other species of Hymenochaetaceae.

【 授权许可】

Unknown