Application of UV-Spectrophotometry and RP-HPLC for Simultaneous Determination of Atorvastatin Calcium and Ezetimibe in Pharmaceutical Dosage Form

Eurasian Journal of Analytical Chemistry

Sandeep S. Sonawane¹  Ravindra A. Fursule¹  Sanjay J. Surana¹  Atul A. Shirkhedkar¹ 

关键词: Spectrophotometry;    Artesunate;    Falcigo;    Safranin O;    Variamine blue;   

DOI  :  10.12973/ejac.2006.00004a

学科分类：分析化学

来源: Eurasian Journal of Analytical Chemistry

【 摘 要 】

Two methods are described for the simultaneous determination of Atorvastatin calcium and Ezetimibe in binary mixture. The first method was based on UV-spectrophotometric determination of two drugs, using simultaneous equation method. It involves absorbance measurement at 232.5 nm (lmax of Ezetimibe) and 246.0 nm (lmax of Atorvastatin calcium) in methanol; linearity was obtained in the range of 5 – 25 mg.mL^-1 for both the drugs. The second method was based on HPLC separation of the two drugs in reverse phase mode using Luna C₁₈ column. Linearity was obtained in the concentration range of 8-22 mg.mL^-1for both the drugs. Both these methods have been successively applied to pharmaceutical formulation and were validated according to ICH guidelines.

【 授权许可】

Unknown