Peripheral endocannabinoids regulate skeletal muscle development and maintenance

European Journal of Translational Myology

Amber Pond¹  Dongjiao Zhao¹  Yefei Wen²  Kevin Hannon¹  Dave Gerrard³  Bruce Watkins⁴  Shihuan Kuang² 

[1] Department of Basic Medical Sciences, Purdue University, West Lafayette, IN;Department of Animal Sciences, Purdue University, West Lafayette, IN;Department of Animal Sciences, Virginia Tech University, Blacksburg, VA;Department of Food Sciences, Purdue University, West Lafayette, IN

关键词: Endocannabinoids;    skeletal muscle;    development;    differentiation;    metabolism;   

DOI  :  10.4081/ejtm.2010.1814

学科分类：骨科学

来源: Pagepress

【 摘 要 】

As a principal tissue responsible for insulin-mediated glucose uptake, skeletal muscle is important for whole-body health. The role of peripheral endocannabinoids as regulators of skeletal muscle metabolism has recently gained a lot of interest, as endocannabinoid system disorders could cause peripheral insulin resistance. We investigated the role of the peripheral endocannabinoid system in skeletal muscle development and maintenance. Cultures of C2C12 cells, primary satellite cells and mouse skeletal muscle single fibers were used as model systems for our studies. We found an increase in cannabinoid receptor type 1 (CB1) mRNA and endocannabinoid synthetic enzyme mRNA skeletal muscle cells during differentiation. We also found that activation of CB1 inhibited myoblast differentiation, expanded the number of satellite cells, and stimulated the fast-muscle oxidative phenotype. Our findings contribute to understanding of the role of the endocannabinoid system in skeletal muscle metabolism and muscle oxygen consumption, and also help to explain the effects of the peripheral endocannabinoid system on whole-body energy balance.

【 授权许可】

Unknown