Histological changes in chronic autoimmune SKG-arthritis evaluated by quantitative three-dimensional stereological estimators

Clinical and Experimental Rheumatology

Frederik Dagnæs-Hansen¹  Shimon Sakaguchi¹  Kristian Stengaard-Pedersen¹  Jens Randel Nyengaard¹  Ellen-Margrethe Hauge¹  Kresten Krarup Keller¹ 

关键词: Bone;    osteoclasts;    cartilage;    histology;    experimental arthritis.;   

DOI  :  

学科分类：医学（综合）

来源: Pacini Editore SpA

【 摘 要 】

OBJECTIVES: To investigate the quantitative arthritic and bone erosive changes, including the number of osteoclasts and osteoclast precursors in the new SKG-model of inflammatory polyarthritis using three-dimensional (3D) stereological methods. METHODS: Arthritis was induced in female SKG-mice with Zymosan A. Quantitative histology was made in four control mice and four mice with arthritis euthanised after 6 and 12 weeks. The right hind paw was embedded undecalcified in methylmethacrylate and cut exhaustively generating vertical uniform random sections. A computer controlled microscope and stereological software was used for histological quantification. Total volumes were estimated according to the Cavalieri principle, total surfaces were estimated using the vertical sections design, and the number of osteoclasts was counted in a physical fractionator. RESULTS: The arthritis score increased during the 12-week period and was paralleled by an increase in the volume of inflammatory tissue (r=0.96, p

【 授权许可】

Unknown