Lack of association between IFNGR1 gene polymorphisms and biopsy-proven giant cell arteritis

Clinical and Experimental Rheumatology

Miguel A. Gonzalez-Gay¹  Rogelio Palomino-Morales¹  Santos Castañeda¹  Tomas Vazquez-Rodriguez¹  Benjamin Fernandez-Gutierrez¹  Jose L. Callejas-Rubio¹  Immaculada C. Morado¹  Orlando Torres¹  Encarnacion Amigo-Diaz¹  Jose A. Miranda-Filloy¹  Javier Martin¹ 

关键词: Giant cell arteritis;    disease susceptibility;    IFNGR1;    genetic studies;    gene polymorphism.;   

DOI  :  

学科分类：医学（综合）

来源: Pacini Editore SpA

【 摘 要 】

OBJECTIVES: Since IFN-gamma plays a pivotal role in the pathogenesis of giant cell arteritis (GCA), a polygenic primary systemic vasculitis involving elderly people from Western countries, in the present study we analysed for first time the implication of three IFN-gamma receptor (IFNGR) 1 gene variants in the susceptibility to and clinical expression of GCA. METHODS: Two hundred and sixteen biopsy-proven GCA patients and 460 matched controls were assessed. DNA from patients and controls was obtained from peripheral blood. Samples were genotyped for three single nucleotide polymorphisms (SNPs) rs1327474 (-611A/G), rs11914 (+189G7C) and rs7749390 (+95C/T) of the IFNGR1 gene using a pre-designed TaqMan allele discrimination assay. Post PCR, the genotype of each sample was attributed automatically by measuring the allelic specific fluorescence on the ABI PRISM 7900 sequence. RESULTS: No significant differences in the genotype or allele distribution between GCA patients and controls for the three IFNGR1 gene variants were found. Furthermore, no significant differences in the genotype distribution were observed when GCA patients were stratified according to the presence of specific clinical features of the disease such as polymyalgia rheumatica or severe ischemic complications including visual ischemic manifestations. CONCLUSIONS: Our results do not show an implication of IFNGR1gene polymorphisms in the susceptibility to and clinical expression of GCA.

【 授权许可】

Unknown