Interleukin-6 gene -174 promoter polymorphism is associated with endothelial dysfunction in patients with rheumatoid arthritis

Clinical and Experimental Rheumatology

Miguel A. Gonzalez-Gay¹  Rogelio Palomino-Morales¹  Tomas Vazquez-Rodriguez¹  Javier Llorca¹  Jose A. Miranda-Filloy¹  Javier Martin¹  Carlos Gonzalez-Juanatey¹ 

关键词: Rheumatoid arthritis;    atherosclerosis;    cardiovascular disease;    gene polymorphism;    cytokine;    IL-6;   

DOI  :  

学科分类：医学（综合）

来源: Pacini Editore SpA

【 摘 要 】

OBJECTIVES:To determine whether the interleukin (IL)6 -174 gene polymorphism may influence the development of subclinical atherosclerosis manifested by the presence of endothelial dysfunction in RA patients.PATIENTS AND METHODS: 311 patients (228 [73.3%] women; 243 [78.1%] rheumatoid factor positive) who fulfilled the 1987 ACR classification criteria for RA seen at the Rheumatology outpatient clinic of Hospital Xeral-Calde, Lugo between March 1996 and December 2006 and 226 matched controls were included in this study. Between March and December 2007, a subgroup of 98 patients randomly selected was assessed for the presence of endothelial dysfunction. Patients and controls were genotyped for a single biallelic (G/C) nucleotide polymorphism (rs1800795) in the promoter region at the position -174 of the IL6 gene using a TaqMan 5´ allele discrimination assay.RESULTS:No significant differences in the IL6 -174 allele or genotype frequency between RA patients and controls were found. However, RA patients homozygous for the IL6 -174 GG genotype had more severe endothelial dysfunction (flow-mediated endothelium-dependent vasodilatation-FMD%: 4.2±6.6) than those carrying the IL6 -174 GC (FMD%: 6.3±8.1) or IL6 -174 CC (FMD%: 6.0±3.3) genotypes. In this regard, significant differences were observed when FMD% values in RA patients carrying the IL6 -174 GG genotype were compared with that observed in those carrying the IL6 -174 GC and the IL6 -174 CC genotypes (FMD%: 6.3±4.6) (p=0.02).CONCLUSIONS:Our results support a role of IL6 -174 gene polymorphism in the development of subclinical atherosclerosis in patients with RA.

【 授权许可】

Unknown