Protein biomarker analysis by mass spectrometry in patients with rheumatoid arthritis receiving anti-tumor necrosis factor-α antibody therapy

Clinical and Experimental Rheumatology

G. Jung¹  M. Yanagida¹  I. Sekigawa¹  K. Iwabuchi¹  S. Sone¹  K. Kaneda¹  Y. Tanaka¹  H. Kaneko¹  K. Takamori¹  H. Ogawa¹  Y. Takasaki¹ 

关键词: Rheumatoid arthritis;    anti-TNF-α antibody;    proteomics;    mass spectrometry;    connective tissue growth factor (CTGF).;   

DOI  :  

学科分类：医学（综合）

来源: Pacini Editore SpA

【 摘 要 】

OBJECTIVES:To investigate the mechanism of action of anti-tumor necrosis factor-α (TNF-α) antibody in patients with rheumatoid arthritis (RA), we analyzed serum or plasma proteins by mass spectrometry system. METHODS:Ten RA patients who received treatment with anti-TNF-α antibody were studied. Samples obtained before and after therapy were analyzed by a two-dimensional liquid chromatography tandem mass spectrometry (2D LC-MS/MS) system after pretreatment by a recently developed method to remove high molecular weight proteins.RESULTS:Using this system, certain proteins were identified after treatment with anti-TNF-α antibody, including proteins related to the TNF-α-mediated pathway for nuclear factor kappa B (NF-κB) activation and/or to the metabolism (including regeneration) of articular cartilage. CONCLUSIONS:Our mass spectrometry system appears to be useful for proteomic analysis. The efficacy of anti-TNF-α antibody therapy for RA may be related to various consequence of the inhibition of TNF-α activity.

【 授权许可】

Unknown