TNF microsatellites polymorphism is associated with rheumatoid arthritis. Confirming evidence in north-western Colombians

Clinical and Experimental Rheumatology

R. Pineda-Tamayo¹  A. Rojas-Villarraga¹  E.A. Ruiz-Narváez¹  J.-M. Anaya¹  L.M. Gomez¹ 

关键词: TNF;    rheumatoid arthritis;    genetic;    microsatellites;    HLA;    Latin America.;   

DOI  :  

学科分类：医学（综合）

来源: Pacini Editore SpA

【 摘 要 】

OBJECTIVES:To examine the contribution of tumor necrosis factor alpha (TNF) microsatellite (a to e) polymorphism to the genetic risk of developing rheumatoid arthritis (RA) in a northwestern Colombian population.METHODS:This was an association study in which 108 RA patients and 222 matched individuals were enrolled. HLA-DRB1 and DQB1 polymorphisms were evaluated to examine for linkage disequilibrium between these loci and TNF micro- satellites. Genotyping was performed using denaturing polyacrylamide gels and polymerase chain reaction-sequence techniques.RESULTS:By unconditional logistic regression analysis, the TNFa6 allele (OR= 2.37, 95%CI 1.07-5.24) and the TNFb4 allele (OR= 3.01, 95%CI 1.07-9.00) were observed to be associated with disease. These associations were independent of HLA-DR and HLA-DQ since linkage disequilibrium between HLA class II and TNF microsatellites was not observed. In addition, patients with the TNFa8 allele had a five times greater risk of developing extra-articular manifestations as compared to patients without this allele (OR = 5.07, 95%CI 1.14 – 22.52), regardless of age and the duration of disease. Haplotype analysis disclosed a protective effect for TNFa7/b7/c1/d4/e3/-308G/-238G.CONCLUSIONS:These results confirm that the TNF locus exerts a primary influence on both susceptibility to and the severity of RA.

【 授权许可】

Unknown