Clinical and Experimental Rheumatology | |
Articular tissues expressing the env-pX transgene are required for generation of arthritogenic T cells in human T cell leukemia virus type I transgenic rats | |
T. Yoshiki1  A. Abe1  H. Ikeda1  Fugo1  K1  A. Ishizu1  | |
关键词: Adoptive transfer; animal model; arthritis; | |
DOI : | |
学科分类:医学(综合) | |
来源: Pacini Editore SpA | |
【 摘 要 】
OBJECTIVES:Human T cell leukemia virus type I env-pX transgenic rats (env-pX rats) were used to investigate the pathogenesis of arthritis.METHODS:Phenotype of cells infiltrated into arthritic joints in env-pX rats was analyzed using flow cytometry and cell-transfer experiments were done using env-pX and wild-type WKAH rats.RESULTS:The majority of T cells infiltrated into arthritic joints in env-pX rats exhibited a CD4 and activated phenotype. Transfer of these T cells into articular space in wild-type WKAH rats succeeded to induce arthritis similarly seen in env-pX rats. However, injection of the cells into sites other than joints did not induce inflammation. Transfer of in vitro-stimulated lymph node cells from disease-free env-pX rats into articular space did not induce arthritis in wild-type WKAH rats.CONCLUSIONS:These findings suggest that articular tissues carrying the env-pX transgene are required for generation of arthritogenic T cells in env-pX rats. However, the constitutive antigens other than the transgene products are recognized as immunological targets by the arthritogenic T cells in the advanced arthritic joints. Molecules expressed specifically in articular tissues may be needed to maintain the inflammatory cell infiltration.
【 授权许可】
Unknown
【 预 览 】
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RO201912020415981ZK.pdf | 670KB | download |