The G protein‐coupled receptor rhodopsin in the native membrane

FEBS Letters

Filipek, Slawomir¹  Palczewski, Krzysztof²  Saperstein, David A²  Fotiadis, Dimitrios³  Liang, Yan²  Engel, Andreas³ 

[1] International Institute of Molecular and Cell Biology, PL-02109 Warsaw, Poland;Department of Ophthalmology, University of Washington, Box 356485, Seattle, WA 98195-6485, USA;M.E. Müller Institute for Microscopy, Biozentrum, University of Basel, Klingelbergstrasse 70, CH-4056 Basel, Switzerland

关键词: Atomic force microscopy;    G protein-coupled receptor;    Membrane protein;    Photoreceptor cell;    Rhodopsin;    Transmission electron microscopy;    AFM;    atomic force microscope/microscopy;    GPCR;    G protein-coupled receptor;    H-I;    helix I;    IC;    intracellular;    ROS;    rod outer segment(s);    TEM;    transmission electron microscopy;   

DOI  :  10.1016/S0014-5793(04)00194-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The higher-order structure of G protein-coupled receptors (GPCRs) in membranes may involve dimerization and formation of even larger oligomeric complexes. Here, we have investigated the organization of the prototypical GPCR rhodopsin in its native membrane by electron and atomic force microscopy (AFM). Disc membranes from mice were isolated and observed by AFM at room temperature. In all experimental conditions, rhodopsin forms structural dimers organized in paracrystalline arrays. A semi-empirical molecular model for the rhodopsin paracrystal is presented validating our previously reported results. Finally, we compare our model with other currently available models describing the supramolecular structure of GPCRs in the membrane.

【 授权许可】

Unknown